All experiments were carried out in triplicate [ 37 ]. All analytical determinations were performed in triplicate. One-way analysis of variance was conducted using SPSS software, Yields of the different extracts are reported in Table 1. Leaf extracts with the different solvents showed higher yields with Ethyl Acetate than with Chloroform.
These yields range from 7. Yield values are influenced by climatic conditions essentially water deficit. The water proportion influences yield for each extract. The presence of various phytochemical constituents in various solvent extracts of the roots is reported in Table 2.
The major constituents present in the root bark of E. The phytochemical screening carried out on the plant powder of the roots shows the presence of phenolic compounds such as flavonoids, anthocyanins, tannins and polyphenols. These roots also contain carbohydrate stores such as mucilage and Oheterosides. Table 2: Phytochemical screening of the root bark powder of Erodium glaucophyllum. These results show that E.
The phenolic compound flavonoid, coumarin, tannin were the principle secondary plant metabolites determined in E. Recently, these compounds have generated considerable interest because their broad pharmacological activities. Anthocyanin helps the human immune system to work more efficiently to protect against viral infections [ 39 ]. Various studies have been demonstrated that coumarin is a potential antioxidant and its antioxidant activity is due to its ability to scavenge free radicals and to chelate metal ions [ 40 ]. Traditionally saponins have been extensively used as detergents, as pesticides and molluscicides, in addition to their industrial applications as foaming and surface active agents and also have beneficial health effects [ 41 ].
It is well known that tannins are used in healing wounds and inflamed mucous membranes [ 42 ]. The Erodium glaucophyllum bark roots Ethyl Acetate extracts are richer in total polyphenols than the Chloroform extracts. TPC varied significantly as a function of extraction solvent as well as the proposition in each solvent.
The Biological Activity of Phytochemicals
These contents reading in Chloroform and Ethyl Acetate extracts Then, the obtained results showed that TPC was dependent on the solvent nature and the equation with water. Suggesting that E. Ethyl Acetate extraction production many contain medium hydrophobic compounds. According to Fratianni et al. The Table 3 shows that the ethyl acetate extracts contains also more flavonoid than the chloroform extracts, with a maximum content Flavonoids play important roles in the development of plants and in the defence against pathogenic germs, predators, UV radiation and environmental stress [ 40 ].
The Chloroform extracts are poorer in tannins than the Ethyl Acetate extracts. The maximum tannin content of the Chloroform extracts and those of Ethyl Acetate are of the order of The antioxidant activities of the roots of E. A good correlation was observed between the TPC of E.
Table 4: Antioxidante activity of two extracts of E. A study by Koleva et al. According to Zimmer et al.
The richness of plant in phenolic compounds explains the higher concentration of inhibition of DPPH whose mechanism depends on the structural conformation of the antioxidant [ 45 ]. According to Miller et al. It is a reactive metal that catalyses oxidative damage in living tissues and cells.
In presence of chelating agent, complex formation is disturbed by the fact that the red color of the complex is decreased [ 46 ]. Meot-Duros et al. The in vitro antibacterial effects of E. The results show that the diameter of the inhibition zone for the extracts reached a maximum of 14 mm for the Gram-negative bacteria and 15 mm for the Gram positive bacteria. Among the Gramnegative bacteria, Salmonella typhimurium appears to be the most sensitive to the chloroform extract; whose inhibition diameter varies from 10 mm to 14 mm.
For the ethyl acetate extracts, the most important antibacterial activity is reported against Listeria monocytogenes by a diameter of inhibition of 15 mm, and against Staphylococcus aureus, Pseudomonas aeruginosaa and Bacillus cereus with an inhibition diameter of 14 mm.
Table 5: Diameters of the inhibition zones of the bacterial growth generated by the various extracts studied. Despite the aridity of the environment, E. This antimicrobial activity can be ascribed to flavonoids, saponins and tannins. These phytochemical groups are known to include anticrobial compound concentration.
Their presence in the plant extracts could therefore explain the observed activity in our study. The phytochemical screening of E. Our study clearly indicates that it is important to measure the anti-oxidant activity using various radicals and oxidation systems and to take both phenolic content and anti-oxidant activity into account while evaluating the antioxidant potential of plant extracts.
Exploitation of these pharmacological properties involves further investigation of these active ingredients by implementation techniques of extraction, purification, separation, crystallization and identification. All Published work is licensed under a Creative Commons Attribution 4.
Visit for more related articles at Journal of Biomedical Sciences. Keywords Erodium glaucophyllum ; Biochemical composition; Antioxidant ; Antibacterial Introduction The medicinal plants appear to be rich in secondary metabolites, widely used in traditional medicine to combat and cure various ailments. Preparation of extract Different extraction conditions have been used type and proportion of the solvent for the preparation of the different extracts of Erodium g.
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